BestKeeper© Software

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity:
BestKeeper - Excel-based tool using pair-wise correlations.



Determination of stable housekeeping genes, differentially regulated target genes
and sample integrity:   BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet, Tanja P. Neuvians

Biotechnology Letters 26:  509-515  (2004)


The stability of standard gene expression is an elementary prerequisite for internal standardisation of target gene expression data and many so called housekeeping genes with assumed stable expression can exhibit either upor down-regulation under some experimental conditions. The developed, and herein presented, software called BestKeeper determines the best suited standards, out of ten candidates, and combines them into an index. The index can be compared with further ten target genes to decide, whether they are differentially expressed under an applied treatment. All data processing is based on crossing points. The BestKeeper software tool was validated on four housekeeping genes and 10 members of the somatotropic axis differentially expressed in bovine corpora lutea total RNA. The BestKeeper application and necessary information about data processing and handling can be downloaded on here.

The paper has been frequently cited by other researchers:  => 1003 times until December 2013

=>  download  BestKeeper version 1  <=
BestKeeper-1.zip

download  WinZip 8.1 


Important note - Password protected files

The BestKeeper Excel spreadsheet ZIP files are password protected.
To get the password by automatic response please contact genequan@wzw.tum.de
or  contact    passwords@gene-quantification.info

By receiving your requested passwords, we will include your e-mail address to our Gene-Qunatification.info and real-time-PCR.info mailing list, and you will receive our monthly appearing Gene Quantification Newsletter called qPCR NEWS and new information about real-time PCR hardware, software and chemistries.


Important note - Computer configurations

In order to be able to run the Relative Expression Software Tool correctly 
in all Microsoft Excel versions and to maintain the macro-virus protection 
for unknown documents as well active, it is recommended to activate 
the 'Trust all installed add-ins and templates' checkbox under

Tools => Macro => Security => Trusted Sources
or
Tools => Macro => Security => Security Level

and to set the 'Security Level' to 'Medium'

as shown in the following two screen shot pictures.


Michael W. Pfaffl (2003)

Determination of most stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper© - Excel© spreadsheet tool using a Repeated Pair-wise Correlation and Regression Analysis
21. Dechema Jahrestagung der Biotechnologen 2.-4. April 2003



Description of  BestKeeper  software

Page 1:  Introduction  &  Input
- real-time PCR Efficiency

Page 2:  Input - of Housekeeping Gene CP Data

Page 3:  Input - of Target Gene CP Data

Page 4:  Output - HKG CP Data &  Repeated Pair-wise Correlation Analysis of HKG

Page 5: Output - Repeated Pair-wise Regression Analysis of HKG

Page 6: Output - HKG CP Data &  Repeated Pair-wise Correlation Analysis of HKG

Page 7: Output - Repeated Pair-wise Correlation and Regression Analysis of TG

Page 8:  Output - Sample Intergrity

Page 9: Output - Graph of HKG CPs

Page 10: Output - Graph of TG CPs


 ©  editor@gene-quantification.info