TATAA Biocenter have found that
the
worldwide demand for training in the field of quantitative
real-time PCR (qPCR) is huge. To coordinate this we aim to arrange
a 3-day qPCR Core Module:
The
first workshop day will be directed to people planning or
considering using qPCR in their research and also users not yet fully
familiar will qPCR.
Second
day targets more advanced users and people concentrating on
different quantification strategies.
The
third day focuses on aspecs in sample preparation and reverse
transcription.
Courses contain both,
theoretical seminars and practical hands-on
training with experienced supervision.
Quantitative
Polymerase Chain Reaction is becoming more and more important in almost
all laboratories. The aim of this international short-course is to give
the participants an in-depth view in the theoretical and practical
knowledge of Quantitative PCR techniques. State-of-the-art experts in
the field of Quantitative PCR techniques from several research and
diagnostic laboratories will provide further theoretical and practical
expertise.
This course is
developed for experienced laboratory technicians or researchers
involved in the life sciences laboratories, incl. medical diagnostics,
microbiology, genetics, pathology, immunology, agriculture,
biochemistry, etc.
Basic
knowledge of molecular biology in general and PCR is a prerequisite. If
you feel you lack the basic knowledge in PCR or the principles of
real-time PCR, but you would like to participate in this quantitative
PCR course, please note that the Avans Hogeschool also offers an
international introductory short course: Principles, parameters, and
applications of PCR (code 2545), which is especially suitable as a
basic introduction to this course.
Course
structure: The course consists
of a series of practical sessions interspersed with lectures and
tutorials. 50% of the time is devoted to lectures and 50% is spent on
"hands-on" experiments, covering techniques that may be directly
applied in research and diagnostic laboratories. All participants will
receive a practical manual containing all used protocols and a
reference manual "Rapid cycle real-time PCR", Springer Verlag, Berlin.
Course
content:
Day 1: Pre-qPCR:
Sample preparation and RNA isolation for qPCR
Day 2: qPCR:
Quantitative PCR techniques (including probelibrary)
The
main focus of the qPCR
2009 Event will
be on Diagnostics & Molecular
Markers
Main
topic: Diagnostics &
Molecular Markers
Markers
in diagnostic, prognostic,
and therapeutic, markers on DNA,
RNA, microRNA, protein, and metabolite level, disease markers, tissue
specific markers, cancer markers, stem-cells markers, differentiation
markers, methylation markers, diagnostic quantification methods,
epigenetics, SNP analysis, HRM = high
resolution melt applications, …..
Main
topic:
Diagnostics & Molecular Markers in agricultural and veterinary
Science
Diagnostics
& Molecular
Markers in White-, Green-, Blue-, and
Yellow- Biotechnology and in agricultural and veterinary Science.
Marker genes on diagnostic, prognostic, and therapeutic markers on on
DNA, RNA, microRNA, protein, and metabolite level, in animals and
plants, … …
RNAi
- microRNA - siRNA
Applications
RNAi
mechanism, microRNA
extraction, qRT-PCR technologies to detect
microRNA, siRNA applications in combination with qRT-PCR, microRNA
targets and microRNA precursors, new siRNA manipulation and microRNA
technologies, .....
High
throughput quantitative PCR
384
well applications, new high
throughput platforms, qPCR robotics,
digital PCR, SNP application, gene expression real-time RT-PCR arrays
(mRNA and microRNA), quantitative multiplexing, …..
qPCR
NOS Session -
Normalization & Optimization & Standardization
new
types of normalization, one
vs. multiple reference genes, genomic
DNA as standard, external standards, optimization of the real-time PCR,
inhibition of negative effects, optimization of real-time PCR
efficiency, qPCR robotics, multiplexing, establishment of DNA / RNA
standards, inter-run standards, national and international studies on
qPCR standardization, new quantification strategies, ........
qPCR
BioStatistics &
BioInformatics
software
applications, data
mining, calculation of relative expression,
primer and probe design on mRNA and microRNA level, real-time PCR
efficiency determination, mathematical modelling, Multivariate
expression profiling raw data analysis, statistics in real-time PCR,
data management, multiway expression profiling, multiple regression
analysis, 3D data visualization, ........
qPCR
Symposium USA 10. -
13. November 2008
Clarion Hotel San Francisco Airport, Millbrae, CA ,
USA
The conference will
be co-located with RNAi Europe. Registered
delegates will have access to both meetings ensuring a very
cost-effective trip.
Make
the most of your trip by attending the pre-conference training course.
Call
for Papers
Deadline:
17 March 2008
If
you would like to be considered for an oral presentation at this
meeting, submit an abstract for review now!
Call
for Posters
Deadline:
4 August 2008
You
can also present your research on a poster while attending to the
meeting. Submit an abstract for consideration now!
The
prominent and still growing place taken by real-time quantitative PCR
in applied and fundamental research and clinical diagnostics almost
appears obvious. However, it is clear that contributions made by
various scientists and companies in the field during the last decade
rendered this technology useful and affordable for many users.
Importantly,
the qPCR domain is still in constant evolution, making it sometimes
hard to stay informed about new methodological approaches or original
studies using the real-time PCR. Therefore, we have scheduled a one day
"Benelux qPCR
Symposium"
on October 6th
2008,
giving the opportunity to the scientific community to get
informed and discuss various aspects of real-time PCR (including but
not limited to new applications, assay optimization and validation, new
technologies, etc.). Scientific talks, posters sessions and industrial
booths will be at the menu => download poster
Sigma-Aldrich
is pleased to be able to bring you a series of webex
seminars dedicated to qPCR. They will include technical
presentations
from international speakers discussing the latest techniques and
aspects of qPCR. To register, go
to QPCR Seminars
Sigma-Aldrich
has the pleasure of inviting you to a seminar series
devoted to the technical aspects of qPCR and RT-qPCR.
Expert
speakers
will cover areas such as:
QPCR assay design and
optimisation
Template handling and
purification
Focus on the
optimisation of RT-qPCR
Various approaches to
genetic analysis
Working with limiting
samples
Data analysis
Troubleshooting
Assay improvement
option for challenging assays
LNA technology and
application
Seminar
grading key:
Level 1 - terrified
and beginners
Level 2 - something
for everyone but a background knowledge is assumed
Level 3 - improvers,
seminars focused on specific areas of interest
Level 4 - brave and
experienced, delving into the fine detail and the more troublesome
aspects of the technique
The Fast Track QPCR
Education Program is a comprehensive program of user training and
advanced education for users of our real-time PCR instruments. The
program provides a wide range of tools to get you up and running with
real-time PCR quickly and effectively. The program also offers you
access to expert Field Application Scientists providing advanced
education. As a leader in the training of scientists on real-time PCR,
we are proud to offer the Fast Track Education Program.
Fast Track QPCR Education
Program Benefits:
Rapid
and effective QPCR training
Advanced
application-focused education
On-demand
educational tools
Ongoing
education to
support new personal and evolving assays
Consultation
with expert Field Application Scientists
Fast Track QPCR Education
Program:
Web-seminars covering
multiple introductory topics. Live web-seminars are provided on a
weekly basis and include time for user questions. Recorded web-seminars
are available in multiple languages.
Register
for and participate in LIVE online seminars presented in
real-time by one of our Technical Services scientists. Also available are online tutorials
and software demonstrations.
Stratagene
offers a variety of live and recorded online seminars
presented by one of our Technical Services Scientists. Simply select a
category of interest below to view the abstracts and to register for a
presentation.
Basic Real-Time PCR training EU
(2 days)
Objective:
In this course, the participant is trained on the basic knowledge of
Real-Time PCR and its applications for quantitative and SNP analysis.
With the use of practical examples, this course focuses on the correct
use of the web-based tools for searching genomic TaqMan. assays and the
SDS and the Primer Express. software. In addition, an overview of the
diverse applications of Real-Time PCR is presented.
This
training course is optimized for scientists new to ABI PRISM. Real-Time
PCR platforms and who wish to establish and optimally use the system
for Real-Time PCR and SNP analysis applications.
