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![]() © BioScience Events - Dr. Michael W. Pfaffl editor@gene.quantification.info Main focus of the GENE
QUANTIFICATION web page is, to describe and summarize all technical
aspects
The GENE QUANTIFICATION web page illustrates the usefulness of reliable quantification strategies, e.g. absolute-, relative-quantification assays, and the difference between them in kinetic PCR & kinetic RT-PCR. RT-PCR is the technique of
choice for analyzing mRNA in extremely low abundance. But real-time
To obtain high fidelity,
accuracy and reliability in reverse transcription and subsequent kinetic
PCR
Main focus of the PHYSIOLOGY
web page is, to describe all kind of kinetic RT-PCR applications in
The reverse transcription - polymerase chain reaction (RT-PCR) is the most sensitive method for the detection of low abundance mRNA, often obtained from limited tissue samples. However, real-time RT-PCR is a complex technique, there are substantial problems associated with its true sensitivity, reproducibility and specificity and, as a quantitative method, it suffers from the problems inherent in RT and PCR. The recent introduction of fluorescence based kinetic (real-time) RT-PCR procedures significantly simplifies the process of producing reproducible quantification of mRNAs and promises to overcome these limitations. Nevertheless, their successful application depends on a clear understanding of the practical problems, and careful experimental design, application and validation remain essential for accurate quantitative measurements of the transcriptom. |
© 2002 - 2004 - editor@gene-quantification.info For further questions concerning any kind of real-time PCR please contact editor@gene-quantification.info optimized for Netscape
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