qPCR Workshops:
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3-days Basic practical
qPCR course @ GeneCore BTU, CAS, BIOCEV
Quantitative polymerase chain reaction (qPCR)
is a vital tool in scientific research due to
its ability to accurately measure and analyse
DNA or RNA samples. It enables precise
quantification of gene expression levels,
providing valuable insights into biological
processes and disease mechanisms.
The Gene Core facility,
in close collaboration with the Laboratory of
Gene Expression, is hosting a comprehensive 3-day
Basic Practical qPCR Course. GeneCore is
a specialized service provider that focuses on
gene expression analysis and its application
in various experiments. With nearly 15 years
of experience in lecturing on qPCR courses, as
well as offering comprehensive training in
Single Cell Analysis, miRNA Analysis, Digital
PCR, and NGS Library Preparation, we have
developed a strong expertise in molecular
biology education and practical laboratory
techniques.
Course schedule:
1.Day:
Introduction to qPCR, Experimental design,
Assay design, 1st Experiment
2.Day: Normalization
and Quantification, Assays validation, 2nd
Experiment
3.Day: Sample
preparation and QC, Reverse transcription,
MIQE guidelines, 3rd Experiment
Lunch, snacks and
coffee are included in the course fee.
Printed course materials are included in the
course fee.
The course fee does not include
accommodation, breakfast, dinner or travel
expenses.
For more information
please contact us at genecore@ibt.cas.cz
or (+420) 325 873 746
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Worldwide Gene Quantification Symposia,
Meetings, and Conferences in the field
of qPCR dPCR & NGS:
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The Ghent
University Consortium for digital PCR is
organizing the European Digital PCR
Symposium and Workshop from 6-8 February
2024 in Ghent
The
one-day symposium (6 February) will
highlight the diverse applications of
digital PCR, including cancer monitoring,
infectious disease quantification and many
more.
The two-day workshop
(7-8 February) will teach you the ins and
outs of digital PCR, including assay and
experiment design, assay optimization,
data analysis, good experimental practice
and data reporting. The course will offer
hands-on sessions supported throughout by
state-of-the-art applications.
Registrations are open (early bird until 22
November)
Oral and poster abstracts are welcome.
The DIGPCR
team,
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3rd International
Symposium on Microgenomics
In the previous editions of the
International Symposium on Microgenomics,
more than 200 people participated in each of
the first two editions in 2014 and 2016
and over 100 during each of our four
2021 webinar series.
Here are a few of their comments:
“Great as well” – “Very good quality
with major scientists” – “Most of them were
excellent and they corresponded to my
expectations”
“Quality was really high” – “The invited
speakers were great” – “High quality of
presentations and the projects presented
were very interesting”
“Perfect” – “Good organization” – “Very good
! Good food and friendly people!”
As in the 2014 and 2016 editions, in the
2023 event, internationally renowned
speakers, experts in their field, will be
presenting an overview
of the present knowledge for obtaining high
quality cells, extracellular vesicles (EV)
or molecules whether in plants, animals or
humans, from agriculture to medicine.
MicroGenomics 2023
New methodologies and strategies for
rare and small sample analysis -- from
agriculture to medicine
Four
scientific sessions:
1) New Methodological
Approaches and Instruments
in MicroGenomics
Analysis
2) Advances in
solid-tissue Analysis
& Spatial-Omics
3)
Circulating Biomarkers -
EVs and Single-Cells
4)
Integrative Analysis -
from multi-omics to
Bioinformatics
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The meeting is
postponed to spring 2024
qPCR & dPCR Under The
Microscope
Harbourview Ballroom, Taronga Centre
Sydney, Australia
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SelectBio
Conferences | Training
Courses | Virtual Events
Upcoming
Conferences and Exhibitions
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qPCR
Seminars and Education Programs:
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Free of
charge web seminars
Sigma-Aldrich is pleased to be
able to bring you a series of webex seminars dedicated to
qPCR. They will include technical
presentations from international speakers
discussing the latest techniques and aspects
of qPCR. To register, go
to
QPCR Seminars
Sigma-Aldrich has the pleasure of
inviting you to a seminar series devoted to
the technical aspects of qPCR and RT-qPCR.
Expert speakers will
cover areas such as:
- QPCR assay design and
optimisation
- Template handling and
purification
- Focus on the optimisation of
RT-qPCR
- Various approaches to genetic
analysis
- Working with limiting samples
- Data analysis
- Troubleshooting
- Assay improvement option for
challenging assays
- LNA technology and application
Seminar grading key:
- Level 1 - terrified and beginners
- Level 2 - something for everyone
but a background knowledge is assumed
- Level 3 - improvers, seminars
focused on specific areas of interest
- Level 4 - brave and experienced,
delving into the fine detail and the
more troublesome aspects of the
technique
Available
Seminars:
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| Author |
Seminar Title |
| Mudassir
Mohammed, Ph.D. |
An
Introduction to qPCR |
| Valerie
Hawkes, Ph.D. |
Assay
Design and Optimisation |
| Neven
Zoric, M.Sc. |
Approaches
to Normal qPCR — Facing the
Challenge of Normalization |
| Tania
Nolan, Ph.D. |
Deriving
a Troubleshooting Protocol |
| Mikael
Kubista, Prof. |
A
Statistical Approach to qPCR Gene
Expression Data Analysis |
| Marina
Tshuikina, Ph.D. |
An
Introduction to Epigenetics Analysis
by qPCR |
| Valerie
Hawkes, Ph.D. |
Improving
the Discrimination and Sensitivity
of qPCR Assays using LNA |
| Chris
Bass, Ph.D. |
Development
of DNA-based Diagnostic Assays for
Sensitive Screening of Mosquito
Disease Vector Populations |
| Michael
Pfaffl, Ph.D. |
Influence
of RNA Integrity on Real-Time RT-PCR
Quantification Data |
| Vladimir
Benes |
microRNA
Profiling |
| Rebecca
Hands |
Technical
Tips on LCM Extraction for mRNA
Profiling |
| Jens
Stolte |
cDNA
Amplification with GenomePlex®
Complete Whole Genome Amplification
Kit |
| Tanya
Novak, M.Sc. |
The
SPUD Assay Has an Important Role in
the Interpretation of Detecting
Pneumocystis DNA in Clinical
Specimens |
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New seminars
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This
six
webinar series on PCR will be based
on MIQE guidelines. MIQE, Minimum
Information for Publication of
Quantitative-Real Time PCR, helps
scientists to design and report
reliable and reproducible qPCR
results. They help others to
understand and replicate already
conducted experiments. In this
webinar series we will discuss step
by step critical points which need
to be taken into consideration in
order to acquire reliable data.
Topics will include:
- Basic principles of
PCR, qPCR and ddPCR
- qPCR Assay Design
- Importance of Sample
Quality in qPCR Analysis
- Improving Assay Quality
by Optimization and Validation
- Data Analysis
- Troubleshooting
See more at: http://www.sigmaaldrich.com/life-science/learning-center/customer-education/pcr-webinar-series.html
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