Hot publications:

• Review - The real-time polymerase chain reaction.
• SPUD -  a quantitative PCR assay for the detection of inhibitors in nucleic acid preparations.
• Review - Real-Time PCR
• Review - Real-Time Reverse Transcription PCR
• Real-time PCR for mRNA quantitation.
• Quantitative real-time RT-PCR – a perspective.
• Relative transcript quantification by Quantitative PCR: Roughly right or precisely wrong ? 
• Linear-After-The-Exponential (LATE)-PCR: Primer design criteria for high yields of specific singlestranded DNA and improved real-time detection.

• Real-time RT-PCR normalisation;   strategies and considerations.
• Validation of oligonucleotide microarray data using microfluidic low-density arrays: a new statistical method to normalize real-time RT-PCR data.
  
• Guideline to reference gene selection for quantitative real-time PCR
• Standardization strategy for quantitative PCR in human seminoma and normal testis.
• Reference gene selection for quantitative real-time PCR analysis in virus infected cells:
• SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections.
• Statistical modeling for selecting housekeeper genes.
• Nomalization of qRT-PCR: a model-based variance estimation approach to identify genes suited for normalization 
• Validation of housekeeping genes for normalizing RNA expression in real-time PCR

Outstanding international publications:

• Fifty Years of Molecular (DNA/RNA) Diagnostics
  
• Helicase-dependent isothermal DNA amplification
• Just add water !  Isothermal amplification kits of lyophilized reagents for field use
• Ramification amplification: a novel isothermal DNA amplification method

Outstanding international publications by Russell Higuchi and coworkers:

• Fifty Years of Molecular (DNA/RNA) Diagnostics
  
• Simultaneous amplification and detection of specific DNA sequences
• Determination of amplification efficiency by dilution and continuous monitoring
• Kinetic PCR analysis: real-time monitoring of DNA amplification reactions
• Kinetic PCR Analysis Using a CCD-camera and without Using Oligonucleotide Probes